[Volver a la
página de inicio]
Institution:
University of Alcala
e-mail:carmen.diazsala@uah.es
Contribution: Expertise in
molecular biology and physiology of forest trees, especially in studies of
developmetal and morphogenic processes involved in forest productivity:
maturation, adventitious rooting and morphogenesis. Library construction,
large-scale gene expression profiling technologies, gene cloning, temporal and
spatial expression of genes and proteins, genetic transformation in Arabidopsis
etc. Large-scale micropropagation and in vitro tissue culture of forest
species. Fully equipped for molecular biology and molecular genetics analysis
at laboratory level including growth chambers and a greenhouse. In addition,
University Central Research Facilities and Equipment include: a sequencing (two
sequencers) facility, a molecular biology unit, a plant production unit, a mass
spectrometry facility, a TEM, SEM and confocal microscopy (and additional equipment),
an isotope unit, a cell culture unit, a X-Ray difraction facility and a NMR
spectroscopy facility.
Participation in several
projects and networks in the field of molecular biology of forest trees and
forest genomics. C. Díaz-Sala is the Spanish representative to the Management
Committee and member of the Steering Committee in Genosilva: European Forest
Genomics Network (COST Action E-28) and chairperson of the COST Action E-28
Working Group2 ”Functional Genomics of Tree Maturation and Reproduction”
Scientists
involved: Carmen Díaz-Sala, Dolores
Abarca, Alicia Solé
Publications
(three):
Day M, Greenwood MS, Díaz-Sala
C. 2002. Age-and size-related trends in woody plant shoot development:
regulatory pathways and evidence for genetic control. Tree Physiology 22:507-513.
Díaz-Sala
C, Garrido G, Sabater B. 2002. Age-related loss of rooting capability in Arabidopsis
thaliana and its reversal by peptides containing the Arg-Gly-Asp (RGD)
motif. Physiologia Plantarum 114:601-607.
Hutchison KW, Singer PB,
McInnis S, Díaz-Sala C, Greenwood MS. 1999. Expansins are conserved in
conifers and expressed in hypocotyls in response to exogenous auxin. Plant
Physiology 120:827-831.
[Volver al
inicio de la página] [Volver a la
página de inicio]
Institution:
University of Córdoba
34071, Córdoba
e-mail:
ir1nacer@uco.es, bf1jonoj@uco.es
Contribution: Expertise in
large-scale protein analysis of forest species by 2D gel-electrophoresis and
mass spectrometry, especially in studies of abiotic stress. Forestation and
field performance of Pinus. Facilities include: greenhouse, cultivate
chamber and ecophysiological equipment (CIRAS 1; Fluorimeter, pressure
chamber), and other basic vegetal biology equipment. Participation in several
European (INCO EU project), and National projects (CICYT), as well as
international Consortiums.
Scientists involved: Rafael Navarro,
Jesús Jorrín
[Volver al
inicio de la página] [Volver a la
página de inicio]
Institution:
University of Girona
17071 Girona
e-mail:
marissa.molinas@udg.es
Contribution: Expertise in
molecular biology, ecophysiology and anatomy of forest trees specially
cork-oak: ecophysiology, anatomy, development, cork and phellem anatomy and
abiotic stress. Library contruction, gene cloning, gene and protein expression
patterns, heterologous expression in yeast and E.coli models. Micro propagation
and repetitive embryogenic lines and tissue culture. Expertise in in vivo
functional assays in E.coli system. Search of candidate genes involved in
phellem development. Fully equiped for histology and molecular biology :
optical and electron microscopy, two automatic sequencers and a Real-time
PCR machine. Participation in serveral projects and networks in forestry and
forest genomics.
Scientists involved: Mercè Figueras,
Gemma Huguet , Dolors Verdaguer and Marissa Molinas
Publications
(three):
Pla M, Jofré A, Martell M, Molinas M,
Gómez J. 2000. Large accumulation
of mRNA
and
DNA point mutations in a plant senescent tissue. FEBS Letters, 472: 14-16.
Puigderrajols P, Jofré A, Mir G, Pla M, Molinas M. 2002. Temporal and spatial distribution patterns of
developmental and stress induced heat shock proteins in cork oak somatic
embryos. J. Expt. Bot. 53 (373): 1-8 (june 2002)
Verdaguer D, Aranda X, Jofré A, El Omari B, Molinas M, Fleck I. 2003. Expression of low molecular
weight heat-shock proteins (sHsps) and total antioxidant activity in the
Mediterranean woody Quercus ilex L. in relation to seasonal and diurnal changes
in physiological parameters. Plant, Cell @ Environmet (in press)
[Volver al
inicio de la página] [Volver a la
página de inicio]
Institution:
University of Malaga
Department of Molecular Biology and Biochemistry
29071 Málaga
(1) Contribution: Expertise in
molecular biology of forest trees, especially in studies of functional genomics
of wood formation and marker development. Library construction, development of
EST collections, large-scale gene expression profiling, gene cloning, temporal
and spatial expression of genes and proteins, heterologous expression, genetic
transformation and reverse genetics in Arabidopsis etc. Fully equipped
for molecular biology and molecular genetics analysis including sequencing
facilities.
Scientists
involved: Francisco R. Cantón,
Concepción Avila.
Publications
(three):
1.- Cantón
FR, Quail PH. 1999. Both phyA and phyB mediate light-imposed repression of
PHYA gene expression in Arabidopsis . Plant Physiol., 121:
1207-1215.
2.- Avila C,
Cantón FR, Barnestein P, Suárez MF, Marraccini P, Rey M, Humara J, Ordás R,
Cánovas FM. 2001. The promoter of a cytosolic glutamine synthetase gene from
the conifer Pinus sylvestris is active in cotyledons of germinating seeds and
light regulated in transgenic Arabidopsis thaliana . Physiologia Plantarum , 112: 388-396
3.- De la Torre F, García-Gutiérrez A, Crespillo R, Cantón FR,
Avila C, Cánovas FM. 2002. Functional expression of two pine
glutamine synthetase genes in bacteria reveals that they encode cytosolic holoenzymes
with different molecular and catalytic properties. Plant Cell Physiol., 43(7):
802-809
(2) Contribution: Expertise in molecular biology of trees (pine and
olive), as well as in bioinformatics. Special attention paid on development of
molecular markers based on unspecifc and specific sequences. Genomic library
constructuion of pine to map nitrogen metabolism genes. Development of
bioinformatics tools to analyse sequences and to store sequences in databases
to extract unigene, clusters, gene families etc. Large-scale gene expression
profiling, gene cloning, temporal and spatial expression of genes and proteins,
heterologous expression, and genetic transformation. Fully equipped for
molecular biology and molecular genetics analysis including sequencing
facilities.
Scientists
involved: M.G. Claros, F.M. Cánovas,
Rocío Bautista, G. Pérez-Trabado
Publications (three):
1.-
S. Funes, E. Davidson, M.G. Claros, R. Van Lis, X. Pérez-Martínez, M.
Vázquez-Acevedo, M.P. King, D. González-Halpen (2002) The tipically
mitochondrial DNA-encoded ATP6 subunit of the mitochondrial F1F0-ATPase
is encoded by a nuclear gene in Chlamydomonas reinhardtii. J. Biol. Chem 277: 6051-6058
2.- R. Bautista, R. Crespillo,
F. M. Canovas, M.G. Claros (2003) Identification of olive-tree cultivars
with SCAR markers. Euphytica 129: 33-41
3.- R. Bautista, F. M.
Canovas, M.G. Claros (2003) Genomic evidence for a repetitive nature of
the RAPD polymorphisms in Olea europaea (olive-tree). Euphytica
in press
(3) Contribution: Expertise in
genetic transformation of forest trees. Biolistic technologies. Analysis and
maintanance of transgenic plants. Fully equipped for molecular biology and
transgenic plant analysis including extensive transgene-secure chamber facilities
and a unit for production of transgenic plants. Capabilities for maintaining
50-60 independently transgenic lines.
Scientists
involved: Fernando Gallardo
Publications
(three):
1.- Gallardo F, Fu J, Cantón FR, García-Gutiérrez A, Cánovas
FM, Kirby EG (1999) Expression of a conifer glutamine synthetase gene in
transgenic poplar. Planta 210:19-26.
2.- Fu J, Sampalo R, Gallardo F, Cánovas FM , Kirby EG
(2003) Assembly of a cytosolic pine glutamine synthetase holoenzyme in leaves
of transgenic poplar leads to enhaced vegetative growth in young plants. Plant
Cell and Environment. In press.
3.-Gallardo F, Fu J, Jing ZP, Kirby EG, Cánovas FM (2003)
Genetic modification of amino acid metabolism in woody plants. Plant Physiology and Biochemistry
Special Issue, In press.
(5) Concepción Ávila Sáez, e-mail: cavila@uma.es
(5) Contribution: Expertise in molecular biology of trees, especially in studies of functional genomics of nitrogen assimilation in conifers. Mainly involved in the study of transcriptional regulation of genes. Isolation of promoters and transient expression studies on pine protoplasts, yeast and heterologous systems, characterization of cis elements and isolation of transfactors.
Scientist involved: Concepción Ávila Sáez
Publications (three):
Gómez-Maldonado J, Cánovas FM and Ávila C. Molecular analysis of the 5' upstream region of a gibberellin-inducible cytosolic glutamine synthetase gene (GS1b) expressed in the pine vascular tissue.Planta(2004) 218: 1036-1045
Gómez-Maldonado J, Ávila C, Barnestein P, Crespillo R and Cánovas. Interaction of cis-acting elements in the expression of a gene encoding cytosolic glutamine synthetase in pine seedlings. Physiologia Plantarum (2004) 121:537-545.
Gómez-Maldonado J, Avila C, de la Torre F, Cañas R, Cánovas FM, and Campbell M. Functional interactions between a glutamine synthetase promoter and MYB proteins. Plant Journal (2004) 39:513-526.
[Volver al
inicio de la página] [Volver a la
página de inicio]
Institution:
Universidad Politecnica de Madrid (1)
28040 Madrid
(1) Contribution: Expertise in
molecular biology of forest trees, especially in studies of wood formation,
cell wall development, biotic and abiotic stress, and tree dormancy. Library
construction, development of EST collections, substracted cDNA libraries, PCR
substration, large-scale gene expression profiling, gene cloning, temporal and
spatial expression of genes and proteins, heterologous expression. Fully
equipped for molecular biology and molecular genetics analysis including
sequencing facilities in the Department.
Scientists
involved: Isabel Allona, Cipriano
Aragoncillo y Rosa Casado
Publications
(three):
1.- I. Allona, M. Quinn, E.
Shoop, K. Swope, S. St. Cyr, J. Carlis, J. Riedl, E. Retzel, M. Campbell, R.
Sederoff y R. W. Whetten. “Analysis of xylem formation in pine by cDNA
sequencing” Proceedings of the National Academy of Science USA, 95: 9693-9698
(1998)
2.-
Y. Zhang, R.R. Sederoff y I. Allona. “Differential expression of genes encoding cell
wall proteins in vascular tissues from vertical and bent loblolly pine trees”
Tree Physiology, 20: 457-466 (2000)
3.-
L. Gomez, I. Allona, R. Casado y C. Aragoncillo. “Seed chitinases” Seed Science
Research, 12:217-230 (2002)
(2) Contribution: Expertise in
genetic analysis of forest trees. Marker development including nuclear
micro-satellites (SSR) as well as PCR derived markers resulting from EST and
gene sequence conversion, to be used in candidate gene approaches. Marker
transfer among species. Construction of saturated linkage maps using dominant
(AFLPs and SAMPLs) and co-dominant markers (SSR and P-ESTs/SNPs).
Candidate-based and neutral marker-based analysis. Comparative and associative
mapping. Population genetics, gene flow and structural analysis of natural
populations. Fully equipped for molecular biology and molecular genetics
analysis, including growth chamber and a greenhouse. Facilities include:
automatic sequencers for fragment analysis, PCR machines, and other basic
molecular biology equipment. In addition, University Central Facilities.
Participation in several
European (TREESNPS I+D EU project) as bilateral (with INRA- France and
University of Davis –USA), and National projects as well as international
Consortiums.
Scientists involved: Carmen Collada
Publications
(three):
1.- Gómez A, González Martínez
SC, Collada C, Climent J and Gil L. 2003. Complex population genetic structure
in the endemic Canary Island pine revealed usig chloroplast microsatellite
markers. Theorical and Applied Genetics (in press)
2.-
García –Casado G, Collada C, Allona I, Soto A, Casado R, Rodríguez Cerezo E,
Gómez L and Aragoncillo C. 2000. Characterization of an apoplastic basis thaumatin-like
protein from recalcitrant chestnut seeds. Physiologia
Plantarum 110: 172-180.
3.-
Sanchez-Monge R, Blanco C, Díaz-Perales A, Collada C, Carrillo T, Aragoncillo C
and Salcedo G. 2000. Class I Chitinases, the panallergens responsible for
the latex-fruit syndrome, are induced by ethylene treatment and inactivated by
heating. J. of Allergy Clin Immunol. 106: 190-195
[Volver al
inicio de la página] [Volver a la
página de inicio]
Institution:
University of Oviedo
33071, Oviedo
e-mail: rrodri@correo.uniovi.es
e-mail: rordas@correo.uniovi.es.
(1) Contribution: Expertise in
epigenetic regulation of tree development. Genomic DNA methylation by High
Performance Capillary Electrophoresis, exon and promoter methylation (MSP), immunodetection
of methylated bases, MSAPs (Methylation-Sensitive Amplification Polimorphisms).
Large-scale in vitro tissue culture of forest trees. Fully equipped for
DNA methylation analysis and tissue culture facilities.
Scientists
involved: Roberto Rodríguez, Maria
Jesús Cañal, Mario F. Fraga, Borja Diego, María Berdasco, Rodrigo Hansbun,
Ricardo Hernández, Carlos Noceda.
Publications (three):
Fraga F. Mario, Uriol
Esther, Diego L. Borja, Berdasco María, Esteller Manel, Cañal María Jesús,
Rodríguez Roberto. 2002. High-performance capillary electrophoresis method for
the quantification of 5-methyl 2-deoxycytidine in genomic DNA: Application to
plant,animal and human cancer tissues. Electrophoresis
23, 1677-1681.
Fraga F Mario, Cañal
María Jesús, Rodríguez Roberto. 2002. Phase-change related epigenetic and
physiological changes in Pinus radiata D. Don. Planta.42, 795-784.
Fraga F Mario, Cañal
María Jesús, Rodríguez Roberto. 2002. Genomic DNA methylation-demethylation during
aging and reinvigoration of Pinus radiata. Tree Physiology 22,813-816.
(2) Contribution: Expertise in plant
development regulation and genetic transformation of forest trees. Large-scale
tissue culture and fully equipped for biochemical and molecular analysis. Biolistic
technologies. Analysis and maintenance of transgenic plants. Fully equipped for
transgenic plant analysis including chamber facilities and capabilities for
maintaining independently transgenic lines.
Scientists
involved: Ricardo Ordás, Manuel Rey,
Victoria González, Mariam López, Belén Fernández, Ana Rodríguez, Mariluz
Centeno.
Publications
(three):
HUMARA, J.M. LOPEZ,M;
ORDAS,R.J. 1999. Agrobacterium tumefacions-mediated transformation of Pinus
pinea L. Cotyledons: an assessment of factors influencing the effficiency
of uidA gene transfer. Plant Cell Reports, 19: 51-58.
HUMARA,
J.M.; MARIN, M.S., PARRA, F.; ORDAS,R.J. 1999. Improved efficiency of uida
gene transfer in Pinus pinea l. cotyledons using a modified binary
vector. Canadian Journal of Forest Research, 73(5):1627-1632.
LÓPEZ, M.; HUMARA,J.M.,
RODRÍGUEZ, R.; ORDÁS, R.J. 2000. Factors involved in Agrobacterium
tumefaciens-mediated gene transfer into Pinus nigra Arn. ssp. salzmannii
(Dunal) Franco. Euphytica114:195-203.
[Volver al
inicio de la página] [Volver a la
página de inicio]
Institution:
University of Salamanca
37007 Salamanca
e-mail:
mdr@usal.es, cnicolas@usal.es
Contribution: Expertise in
molecular biology and physiology of forest trees, especially in studies of seed
dormancy and germination. Library construction, gene cloning, temporal and
spatial expression of genes and proteins, heterologous expression, genetic
transformation etc. Fully equipped for molecular biology and molecular genetics
analysis including growth chambers, sequencing and confocal microscopy
facilities.
Participation in several
projects in the field of molecular biology of forest trees.
Scientists
involved: Dolores Rodríguez, Carlos
Nicolás
Publications (three):
Lorenzo
O, Nicolás C, Nicolás G, Rodríguez D (2002). GA3-induced
expression of a new functional AAA-ATPase (FsA1) is correlated with the onset
of germination in Fagus sylvatica L. seeds (beechnuts). Plant Cell
Physiol 43: 27-34
Lorenzo, O., Nicolás, C.,
Nicolás, G. and Rodríguez, D. (2002) Molecular cloning of a functional protein
phosphatase 2C (FsPP2C2) with unusual features and synergistically up-regulated
by ABA and calcium in dormant seeds of Fagus sylvatica. Physiol Plant
114: 482-490.
Lorenzo, O., Rodríguez, D., Nicolás, G., Rodríguez, P.L.
and Nicolás, C. (2001) A new protein phosphatase 2C (FsPP2C1) induced by
abscisic acid is specifically expressed in dormant beechnut seeds. Plant Physiol 125: 1949-1956
GRUPO
BIOLOGÍA DE SEMILLAS. DEPARTAMENTO DE FISIOLOGÍA VEGETAL. UNIVERSIDAD DE
SALAMANCA:
Plantilla:
Gregorio
Nicolás Rodrigo
Dolores
Rodríguez Martín
Carlos
Nicolás Rodríguez
Becarios:
Mary
Paz González García
David
Reyes Martín
PUBLICACIONES
AUTORES : C. NICOLAS, G. NICOLAS, D. RODRIGUEZ.
TÍTULO: “Transcripts of a
gene, encoding a small GTP-binding protein from Fagus sylvatica, are
induced by ABA and accumulated in embryo axis of dormant seeds.”
REF.
REVISTA/LIBRO: Plant Mol. Biol.
36:487-491.
1998.
CLAVE: A
AUTORES : C. NICOLAS, J.M. dePRADA, O. LORENZO, G. NICOLAS,
D. RODRIGUEZ.
TÍTULO: “Abscisic acid
and stress regulate the expression of calmodulin in germinating chick-pea
seeds.”
REF.
REVISTA/LIBRO: Physiol. Plant. 104:
379-384.
1998.
CLAVE: A
AUTORES : O. LORENZO, D. RODRIGUEZ, C. NICOLAS, G. NICOLAS.
TÍTULO: “Characterization
and expression of two protein kinase and an EIN3-like genes, which are regulated
by ABA and GA3 in dormant Fagus sylvatica seeds”.
REF. REVISTA/LIBRO: “Seed Biology:
Advances and Applications”. Eds. M. Black, K.J. Bradford and J. Vázquez-Ramos. Pg. 329-340. 2000.
EDITORIAL: CAB
International
CLAVE: CL
AUTORES: D. RODRIGUEZ, O. LORENZO, C. NICOLAS.
TÍTULO:“El ácido abscísico y la dormición de semillas.”.
REF.
REVISTA/LIBRO: “Fisiología Hormonal”.
Ed. A. Matilla. Pg, 41-51. 2000
EDITORIAL:
Servicio de
Publicaciones de la Universidad de Santiago de
Compostela
CLAVE: R
AUTORES : O. LORENZO, D. RODRIGUEZ, G. NICOLAS, PL RODRIGUEZ,
C. NICOLAS.
TÍTULO: “A new protein
phosphatase 2C (FsPP2C1) induced by ABA is specifically expressed in
dormant beechnut seeds.”.
REF.
REVISTA/LIBRO: Plant Physiol 125:
1949-1956.
2001.
CLAVE: A
AUTORES : O. LORENZO, C. NICOLAS, G. NICOLAS, D
RODRIGUEZ.
TÍTULO: “Molecular
cloning of a functional protein phosphatase 2C (FsPP2C2) with unusual features
and synergistically up-regulated by ABA and calcium in dormant seeds of Fagus
sylvatica”.
REF.
REVISTA/LIBRO: Physiol Plant 114:
482-490
2002 . CLAVE:
A
AUTORES : O. LORENZO, C. NICOLAS, G. NICOLAS, D
RODRIGUEZ.
TÍTULO: “GA3-induced
expression of a new functional AAA-ATPase (FsA1) is correlated with the onset
of germination in Fagus sylvatica L. seeds (beechnuts)”.
REF. REVISTA/LIBRO: Plant and Cell
Physiology 43:27-34. 2002.
CLAVE: A
AUTORES : MP GONZÁLEZ, D RODRÍGUEZ, C NICOLÁS, PL RODRÍGUEZ,
G NICOLÁS, O LORENZO
TÍTULO: Negative
regulation of ABA-signaling by the Fagus sylvatica FsPP2C1 plays a role
in seed dormancy regulation and promotion of seed germination
REF.
REVISTA/LIBRO: Plant Physiol (en
prensa)
CLAVE: A
AUTORES : AP CALVO, JA JIMÉNEZ, C NICOLÁS, G NICOLÁS, D
RODRÍGUEZ
TÍTULO: Isolation and
characterization of genes related with the breaking of beechnuts dormancy and
putatively involved in ethylene signal perception and transduction
REF. REVISTA/LIBRO: The Biology of
Seeds: Recent Research Advances. Eds: G Nicolás, KJ Bradford, D Come, HW
Pritchard. (en prensa)
EDITORIAL: CAB
International
CLAVE: CL
AUTORES : O. LORENZO, C. NICOLAS, G. NICOLAS, D
RODRIGUEZ.
TÍTULO: Characterization
of a dual plant protein kinase (FsPK1) up-regulated by ABA and calcium and
specifically expressed in dormant seeds of Fagus sylvatica L.
REF.
REVISTA/LIBRO: Enviado a Seed Sci
Res
.
CLAVE: A
AUTORES : LC MORTENSEN, D. RODRÍGUEZ, G. NICOLÁS, EN ERIKSEN,
C NICOLAS
TÍTULO: Changes in a
seed-specific abscisic acid-responsive glycine rich protein (GRPF1) mRNA may
reflects the release of seed dormancy in Fagus sylvatica during moist
prechilling
REF.
REVISTA/LIBRO: Enviado a Seed Sci
Res
.
CLAVE: A
TITULO
DEL PROYECTO : Estudio de la
dormición en semillas forestales: Papel regulador del ácido abscísico, ácido
giberelico y etileno en la dormición de semillas de Fagus sylvatica y Pseudotsuga
menziesii.
ENTIDAD
FINANCIADORA DGES
IDENTIFICACION: PB96-1313
DURACION; 1997 HASTA; 2000
INVESTIGADOR
PRINCIPAL: Gregorio Nicolás Rodrigo
NUMERO
DE INVESTIGADORES PARTICIPANTES: 4
IMPORTE
TOTAL DEL PROYECTO: 15.005.000 pts
TITULO
DEL PROYECTO : Regulación de la
dormición de semillas de haya (Fagus sylvatica) por ácido abscísico,
giberelinas y etileno.
ENTIDAD
FINANCIADORA: Junta de Castilla y
León.
IDENTIFICACION
: SA60/99
DURACION; 1999 HASTA; 2001
INVESTIGADOR
PRINCIPAL: Mª Dolores Rodríguez
NUMERO
DE INVESTIGADORES PARTICIPANTES: 5
IMPORTE
TOTAL DEL PROYECTO: 3.095.000 pts
TÍTULO: Caracterización de la dormición de semillas de Fagus
sylvatica. Regulación del proceso por etileno, ácido abscísico y ácido
giberélico
ENTIDAD: Ministerio de Ciencia y Tecnología. (BFI2000-1361)
DURACION,
DESDE:
2000 HASTA:
2003
INVESTIGADOR
RESPONSABLE: Dr. Gregorio Nicolás
Rodrígo
NUMERO
DE INVESTIGADORES PARTICIPANTES: 3
IMPORTE
TOTAL DEL PROYECTO: 21.000.000 pts
TÍTULO: Caracterización y expresión de proteína fosfatasas
(PP1, PP2A y PP2C) relacionadas con la dormición de semillas de haya (Fagus
sylvatica L.)
ENTIDAD: Junta de Castilla y León. (SA010/02)
DURACION,
DESDE:
2002 HASTA:
2004
INVESTIGADOR
RESPONSABLE: Dr. Carlos Nicolás
Rodríguez.
NUMERO
DE INVESTIGADORES PARTICIPANTES: 4
IMPORTE
TOTAL DEL PROYECTO: 19.423 EUROS
TESIS
DOCTORALES DIRIGIDAS
TITULO: “Regulación hormonal de la dormición de semillas de
haya (Fagus sylvatica): Expresión de proteínas específicas relacionadas con la
transducción de señales.”
DOCTORANDO: OSCAR LORENZO SANCHEZ
UNIVERSIDAD:
Salamanca
FACULTAD/ESCUELA: F. de Biología
DIRECTOR/ES:
Dra. D.Rodríguez y Dr. C. Nicolás
AÑO:
2000
CALIFICACION: Apto cum laude
TITULO: “Regulación de la dormición de semillas de haya
(Fagus sylvatica): aislamiento y caracterización de clones implicados en la
biosíntesis de etileno y giberelinas y en las respuestas a estas hormonas.”
DOCTORANDO:
ANGEL PABLO CALVO BULLON
DIRECTOR/ES:
Dr. G. Nicolás y Dra.
D.Rodríguez
UNIVERSIDAD:
Salamanca
FACULTAD/ESCUELA: F. de Biología
AÑO:
2002
CALIFICACION: Sobresaliente cum laude
TÍTULO: "Caracterización bioquímica y molecular de la
1-aminociclopropano-1-carboxílico y la endopoligalacturonasa en semillas de
nabiza (Brassica rapa L. cv. Rapa).
DOCTORANDO: MARÍA DEL CARMEN RODRÍGUEZ GACIO
DIRECTOR/ES:
Dra. A. Matilla y Dr. C. Nicolás
UNIVERSIDAD: Santiago de Compostela FACULTAD/ESCUELA:
F. de Farmacia
AÑO:
2002
CALIFICACION: Sobresaliente cum laude
TITULO: “Regulación por ácido abscísico y giberelinas de la
expresión de proteínas relacionadas con la dormición de semillas de haya (Fagus
sylvatica L.)”.
DOCTORANDO: JESUS ANGEL JIMENEZ NIETO
UNIVERSIDAD:
Salamanca
FACULTAD/ESCUELA: F. de Biología
DIRECTOR/ES:
Dra. D.Rodríguez y Dr. C. Nicolás
AÑO: Fecha lectura aproximada,
2003 CALIFICACION:
En realización.
TITULO: “Regulación de la expresión de proteín-fosfatasas
relacionadas con la dormición de semillas de haya (Fagus sylvatica L.)”.
DOCTORANDO: Mª PAZ GONZALEZ GARCIA
UNIVERSIDAD:
Salamanca
FACULTAD/ESCUELA: F. de Biología
AÑO: Fecha lectura aproximada,
2004 CALIFICACION:
En realización.
[Volver al
inicio de la página] [Volver a la
página de inicio]
Institution:
University of Valencia
46100 Valencia
e-mail:
isabel.arrillaga@uv.es
Contribution: Expertise in
large-scale tissue culture. Protocols to in vitro propagation of tree species
(Sorbus domestica, Fraxinus ornus, Robinia pseudoacacia, Juniperus oxycedrus,
Cedrus libani and Cedrus atlantica). Expertise in genetic transformation of
forest trees. Biolistic technologies. Analysis and maintanance of transgenic
plants. Two fully equipped laboratories for molecular biology and transgenic
plant analysis including extensive transgene-secure chamber facilities.
Capabilities for maintaining independently transgenic lines (in vitro or in the
greenhouse). In vitro tests of stress tolerance. In addition,
sequencing, TEM, SEM microscopy, and mass spectrometry facilities for
supporting other groups involved in the project.
TRANSGENIC LINES AVAILABLE
- Herbicide tolerant Robinia
pseudoacacia (black locust) transgenic lines
- Transgenic lines of the
aromatic shrub Lavandula latifolia
- Transgenic tobacco lines
Scientists involved: Isabel Arrillaga,
Juan Segura and Jesus Muñoz
Publications
(three):
- Gisbert C, Rus AM, Bolarin
MC, Coronado JM, Arrillaga I, Montesinos C, Caro M, Serrano R, Moreno V. 2000. The yeast HAL1 gene
improves salt tolerance of transgenic tomato. Plant Physiology, 123: 393-
402.
- Nebauer SG, Arrillaga I,
del Castillo-Agudo L, Segura J. 2000. .Agrobacterium tumefaciens-mediated
transformation of the aromatic shrub Lavandula latifolia: Long-term
stable transgene expression and segregation in the progeny: Molecular Breeding,
6: 539-552.
-
Zaragozá C; Muñoz, J Arrillaga I. 2003. Regeneration of herbicide
tolerant black locust transgenic plants by the SAAT method. (under revision)
-
Sales E, Segura J, Arrillaga I. 2003. Agrobacterium tumefaciens-mediated genetic
transformation of the cardenolide-producing plant Digitalis minor L.
Planta Medica. 69: 143-147
[Volver al
inicio de la página] [Volver a la
página de inicio]
Institution:
University of Valladolid
Escuela Técnica Superior de Ingenierías
Agrarias
Department of Plant Production and Silvopasciculture
Avda de Madrid,44
34071, Palencia
Contact Person: Rosario
Sierra
e-mail:
rsierra@pvs.uva.es
Contribution: Expertise in genetics and improvement of forest
trees.
Large-scale tissue culture, development of biochemical and molecular markers.
Fully equipped for tissue culture, biochemical and molecular analysis.
Scientists involved: Rosario Sierra, Elena Hidalgo, Dolores Cristóbal,
Teresa Martín.
Publications (three):
Hidalgo, E.; Palacios,
JM.; Murillo, J. y Ruiz-Argüeso, T . "Nucleotide sequence and characterization
of four additional genes of the hydrogenase structural operon from Rhizobium
leguminosarum".
M.T. Martin, M.T. Cervera & J.A. Garcia Properties of the active
plum pox potyvirus RNA polymerase complex indefined glycerol gradient
fractions.
Virus Res. 37, 127-137. (1995)
Sierra-de-Grado, R.; Moulia,
B.; Fournier, M.; Alía, R.; Díez-Barra, R. Genetic control of stem form in
Pinus pinaster Ait. seedlings exposed tolateral light. Trees, Structure and
Function 11:455-461.(1997)
[Volver al
inicio de la página] [Volver a la
página de inicio]
Institution:
University of Vigo
33071 Vigo
University of
Santiago de Compostela
Department of Plant
Physiology
27002 Lugo
e-mail:
mrey@uvigo.es
Contribution: Expertise in
genetic transformation and molecular biology in woody species, especially in
PCR-based marker development (RAPD and AFLP), their conversion into SCAR-type
markers, and in gene expression profiling technology (cDNA-AFLP). Maintenance
of transgenic lines and molecular characterization and gene expression analysis
of transgenic plants. Laboratory facilities include basic molecular biology
equipment, growth chambers and greenhouse, and real-time PCR machine
Universities central research facilities include microscopy services (TEM, SEM,
and confocal microscopy), mass spectrometry facilities, proteomic services and
extensive growth chambers.
Scientists involved: Manuel Rey, Mª
Victoria González, Mª Ángeles López
Publications
(three):
Rey, M.; González, M.V.;
Ordás, R.J.; Ancora, G.; Tavazza, R. 1996. Factors affecting transient gene
expression in cultured radiata pine cotyledons following particle bombardment. Physiologia
Plantarum 96: 630-636.
López, M.; Humara, J.M.;
Rodríguez, R.; Ordás, R.J. 2000. Transient uidA gene expression in
electroporated cotyledonary protoplasts of Pinus nigra Arn. subsp.
salzmannii (Dunal) Franco and in bombarded cotyledons. Canadian Journal of
Forest Research 30: 448-455.
Ávila, C.; Cantón, F.R.;
Barnestein, P.; Suárez, M.-F.; Marraccini, P.; Rey, M.; Humara, J.M.; Ordás,
R.; Cánovas, F.M. 2001. The promoter of a cytosolic glutamine synthetase gene
from the conifer Pinus sylvestris is active in cotyledons of germinating
seeds and light-regulated in transgenic Arabidopsis thaliana.
Physiologia Plantarum 112: 388-396.
[Volver al
inicio de la página] [Volver a la
página de inicio]
e-mail:
conchi@cesga.iiag.es
Contribution: Expertise in
molecular biology of forest trees, especially in studies of phase change,
adventitious rooting and morphogenic capacity of adult tissues. Library
construction, gene cloning, temporal and spatial expression of genes and
proteins. Genetic transformation of forest species and analysis of transgenic
lines. Large scale micropropagation of forest trees and in vitro tissue culture
facilities. Plant regeneration by adventitious buds and somatic embryogenesis
in different forest tree species. Fully equipped for molecular biology and
molecular genetics analysis, including microscopy and histology facilities.
Participation in different
National projects in the field of molecular biology, genetic transformation,
and plant regeneration by somatic embryogenesis.
Scientists
involved: Concepción Sánchez, Antonio
Ballester, Ana Mª Vieitez.
Publications
(three):
Gil B, Pastoriza E, Ballester A, Sánchez C. 2003. Isolation
and characterization of a cDNA differentially expressed in juvenile-like and
mature shoots of Quercus robur. Tree Physiology. In press.
Corredoira E, Vieitez AM,
Ballester A. 2002. Somatic embryogenesis in elm. Annals of Botany, 89:
637-644.
Corredoira E, Ballester
A, Vieitez AM. 2003. Proliferation and maturation of Castanea sativa
somatic embryos originated from leaf explants. Annals of Botany. In press.
[Volver al
inicio de la página] [Volver a la
página de inicio]
Department of Forest Systems and Resources
Ctra. La Coruña. 28040, Madrid
(1) Unit of Forest Genetics and Ecophysiology:
Genomics: Maria Teresa Cervera e-mail: cervera@inia.es
Population Genetics: Santiago González-Martínez. e-mail: santiago@inia.es
Quantitative Genetics: Ricardo Alía. e-mail: alia@inia.es
Ecophysiology: Ismael Aranda. e-mail: aranda@inia.es(2) Unit of Biotechnology
Mª Angeles Bueno e-mail: bueno@inia.es
(
(1) Contribution:
Genomics:Analysis of pine adaptation to drought stress: Identification of genes involved in response to drought stress (construction of EST, SSH libraries), construction of functional linkage maps. Comparative and association analysis. QTL analysis. Marker development including nuclear micro-satellites and gene-based markers.
Participation in several European (TREESNPS R&D EU project; EVOLTREE NoE), bilateral (with INRA-France and University of Göttingen–Germany), and National projects as well as international Consortiums.
Scientists involved: María Teresa Cervera, M. Ángeles Guevara, Nuria de María, Carmen Collada (Joint Unit INIA-UPM) and Álvaro Soto (Joint Unit INIA-UPM).
Publications (three):
Cervera M-T, Storme V, Liu B, Steenackers M, Ivens B, Michiels B, Gusmao J, van Montagu M and Boerjan W. 2001. Dense genetic linkage maps of three Populus species (P. deltoides, P. nigra and p. trichocarpa) based on AFLP, and microsatellite markers. Genetics 158: 787-809.
L Gil; P Fuentes-Utrilla; A. Soto; M.T. Cervera; C. Collada. 2004. English elm is a 2.000-year-old Roman clone. Nature 431: 1053-1053
S.C. González- Martínez, J. J. Robledo-Arnuncio, C. Collada, A. Díaz, C. G. Williams, R. Alía, M.T. Cervera. 2004. Cross-amplification and sequence variation of microsatellite loci in Eurasian hard pines. Theoretical and Applied Genetics 109: 103-111
(2) Contribution: Expertise in
Biotechnology, Genetics and improvement of forest trees, specially in
organogenesis and embryogenesis. Large-scale micropropagation, rooting,
response to stress, maturation, dormancy, germination, genetic transformation
and development of neutral-based markers for analysing plant quality. Genetic
analysis of forest trees by isozyme, RAPD and microsatellite markers,
characterisation, population genetics and quantitative genetics. Induction of
haploidy, double haploids and homozygous plant lines by anther, microspore and
pollen culture. Fully equipped laboratory for tissue culture and genetic
characterisation, including two laminar flow cabinets (1 vertical flow, 1
horizontal), fluorescence microscope, flow cytometer, PCR termocycler,
electrophoresis equipment, growth chamber facilities, greenhouse and
capabilities for maintaining independently transgenic/cultured lines.
Participation in several projects and networks in the field of Biotechnology of
forest trees and forest genetics at European (FOREST I+D EU projects: Upgrading
of Southern Pines; Adaptation and Selection of Mediterranean Pinus and Cedrus
for sustainable Afforestation of marginal lands; European Research on cork oak
and cork), bilateral (with BOKU-Vienna, Austria, and the Institut für
Obstzüchtung of Dresden–Germany), and National projects as well as
international Consortiums: Biotechnological approach for utilisation of gametic
cells (European COST Action 824), and co-ordinator of several research
projects.
Scientists involved: M.A. Bueno,
José Antonio Manzanera
Publications
(three):
BUENO M.A, GOMEZ A, MANZANERA
J.A. Propagation and DNA markers characterization of Populus tremula L
and Populus alba L. 2003 IN: Jain SM, Ishii K, (eds.). Micropropagation
of Woody Trees and Fruits. Forestry Sciences. Vol. 75, 37-74.Kluwer Academic
Publishers. Dordrecht. The Netherlands ISBN 1-4020-1135-0
BUENO M.A, MANZANERA
J.A. Oak anther culture. 2003 IN: Maluszynski M, Kasha K.J , Foster B.P. and
Szarejko I (Eds). Doubled Haploid Production in Crops Plants. A Manual. Kluwer
Academic Publishers, Dordrecht. Vienna.
GÓMEZ, A., PINTOS, B.,
AGUIRIANO, E., MANZANERA, J.A., BUENO, M.A. 2001 SSR markers for Quercus
suber tree identification and embryo analysis. Vol 92 (3). 292-295. J. of
Heredity.
[Volver al
inicio de la página] [Volver a la
página de inicio]
01080
Alava
e-mail:
eritter@neiker.net
Contribution:
NEIKER is a public research
institute depending on the Basque Government in Northern Spain. The institute
consists of three departments carrying out basic and applied research in
agriculture (plants and animals). Between 100 to 120 scientists,
technical assistants and other staff members, including undergraduate and
graduate students are active in the three departments and administration.
NEIKER is involved in general
agricultural research including forest species. Pinus radiata represents
the predominant species in the productive forests of northern Spain. The
scientific staff is experienced in forest breeding and is responsible for
the ongoing local forest breeding program.
Molecular techniques are well established at
the institute. Within other national and EU projects different biotechnological
techniques were developed and are optimised continuously for forest species but
also for other crop species. These techniques include all kinds of PCR and
hybridisation based techniques, electrophoretic techniques, sequencing, cloning
and others.
Particularly, NEIKER has cordinated the related
EU project FAIR3 CT96-1445 "Development, validation and application of
molecular, morphological and physiological markers for juvenile and mature
state characterization in woody plant species" (see http://www.neiker.net/PhaseDB
for details) in which different marker types and indicators for phase changes
(juvenil-adult; vegetative-generative) were developed which can be used for
predicting rooting capacities or flowering behaviour. Currently, NEIKER
coordinates the EU project QLK5-1999-01159:
"Construction and application of a multifunctional and saturated genetic
map for coniferous species".
From
this project an ultra-high density linkage map (UHD map) of Pinus
pinaster based on nearly 2000 AFLP markers and numerous EST and SSR markers
is available. This map is exploited to full extent for comparative genome
and QTL analysis in different genetic backgrounds. Other published linkage maps
in forest species have been partially aligned with the reference map. Satellite
maps based on a common subset of AFLP primer combinations and EST and SSR
markers have been obtained in this project for P. radiata, P.
sylvestris, Picea abies and Pseudotsuga menziesii, allowing
to perform comparative genome analysis in different pine species and related
gymnosperms. QTL analysis for important characters such as growth traits, wood
quality and physiological characters have been performed in reference and
satellite mapping populations. Allele-specific markers for important QTLs have
been developed and their variability in local germplasm and breeding material
of different forest species is currently analysed (see http://www.neiker.net/UHDfor for
details).
Within the frame of the EU-Project
QLK5-CT2002-01849 "Developing a physical and functional map of potato:
creating new sources for molecular markers to breed cultivars with multiple
resistances and quality traits (APOPHYS)" and a National Project
RTA03-001-C2 "Obtención de un mapa genético, físico y funcional y
detección de genes para estrés biótico y abiótico en la vid (GENOVID)",
transcriptome maps are being constructed in potato and grapewine, respectively.
On the other hand differential cDNA display and candidate gene expression
related to drought resistance is currently being performed in these species
within the mentioned projects.
Scientists involved: Enrique Ritter,
Santiago Espinel, Pablo Goicoetchea, Ana Aragonés.
Publications
(three):
Ritter E; Aragones A;
Markussen T; Acheré V; Espinel S; Fladung M, Wrobel S; Faivre-Rampant P; Jeandroz S; Favre JM (2002). Towards
construction of an ultra high density linkage map for Pinus pinaster.
Annals of Forest Science 59: 637-643.
Markussen, T., Fladung, M.,
Achere, V., Favre, J.M., Faivre-Rampant, P., Aragones, A., Da Silva
Perez, D., Harvengt, L., Espinel, S., Ritter, E. (2003) Identification of QTLs
controlling growth, chemical and physical wood property traits in Pinus
pinaster (Ait.) Silvae Genetica (in press)
Ritter E, , Espinel S, Fladung M, Favre JM (2003).
High
density linkage maps in conifer species and their potential application. In:
Molecular Genetics and Breeding of Forest Trees. Eds: Kumar S &
Fladung M., The Haworth Press Inc. (in press)
[Volver al
inicio de la página] [Volver a la
página de inicio]